Instrument Applications

The Agilent Seahorse XF platform measures the two main metabolic pathways, mitochondrial respiration and glycolysis, for live cells in real time, to provide functional kinetic measurements of cellular bioenergetics. Discover how bioenergetic parameters can provide powerful insights and can serve as an indicator for disease models, critical cell processes, and therapeutic discoveries. 

Assessing the Mitochondrial Toxicity of Environmental Pollutants Using the Agilent Seahorse XF Extracellular Flux Analyzer 

A Customized XF Workflow for Detection and Characterization of Mitochondrial Toxicity 

Gaining Insights into Disease Biology for Target Identification and Validation 

Immune Metabolism 

Immune cell processes including activation, proliferation, and memory development are all driven by metabolic reprogramming, which can be tuned to enhance performance and control immune cell outcomes. Discover insights into immune cell processes including activation, proliferation, and memory development through functional real-time metabolic measurements. 

Improved CAR-NK Metabolic Fitness Leads to More Persistent Killing and is Predictive of Superior Clinical Outcomes 

Assessing T Cell Bioenergetic Poise and SRC Using Extracellular Flux Analysis 

Cancer Metabolism 

Metabolism is a critical driver of cancer malignancy, with cancer cell proliferation commonly requiring an upregulation or “metabolic switch” towards a more glycolytic phenotype to fuel the rapid energy requirements and generate building blocks for cancer cell growth. Reveal cancer metabolic liabilities through functional bioenergetic analysis of live cells in real time for a deeper understanding of cancer cell biology. 

Rapid Bioenergetic Functional Screening of Anticancer Drug Candidates 

xCELLigence RTCA eSight system

Immune Cell Killing Assays 

Typical immune cell killing assays are labor-intensive and require users to collect multiple timepoints using a combination of techniques. In contrast, the label-free xCELLigence RTCA instruments provide a complete view of immune cell killing and closely mimics activity in vivo. Obtain highly sensitive, reproducible, and direct measurements of immune cell killing potency in real time. This simple and high throughput workflow also provides greater insight into cellular mechanism of action, serial killing, and exhaustion. 

Optimizing Pulse Label Fluorescent Label Conditions and Image Acquisition with eSight 

Bispecific T Cell Engager (BiTE) Mediated Cancer Cell Lysis Assay 

A Novel Real-Time Co-Culture Assay Using xCelligence RTCA eSight for Immune Cell Invasion and Cytotoxicity 

Metabolic Preconditioning Improves Engineered T Cell Fitness and Function 

 
Viral Cytopathic Effect Assays 

Advance your anti-viral therapy or vaccine development by accurately measuring viral cytopathic effects (CPE) in real time with Agilent xCELLigence RTCA instruments, without the use of labor-intensive plaque assays. Monitor viral CPE automatically, from minutes to days, and greatly reduce workload and manual handling of samples. Measure cell proliferation kinetics, identify the optimal time point for viral infection with different cell seeding densities, and assess virus-mediated cytopathogenicity in one workflow. 

Real-Time Antibody Internalization Assays with Agilent xCELLigence RTCA eSight 

Cytotoxicity Assays 

Determine cytotoxic effects of drugs or compounds by monitoring cell proliferation, cell size or morphology, and cell-substrate attachment quality in real time. Monitor the kinetics of long-term cellular responses with this simple and high-throughput cytotoxicity assay. Make informed decisions about the timing of cell treatments and easily generate dose-response curves at multiple time points, without increasing the workload. 

Monitoring Drug-Mediated 3D Tumor Spheroid Shrinkage Using xCELLigence RTCA eSight 

 
Apoptosis and Proliferation Assays 

Learn about apoptosis and proliferation assays that are simple, quantitative, and high throughput to provide multiple perspectives into cell health and viability. Measure real-time cell health directly and capture early and late apoptotic events such as modulation of cell adhesion, cell membrane integrity, and cell detachment. Track proliferation of host cells to detect readout for drug toxicity or presence of a virus. Detect and quantify apoptosis in living cells by label-free approach, or using apoptotic or nuclear labeling reagents. The xCELLigence RTCA eSight provides multiple temporal vantage points (impedance, fluorescence imaging, and brightfield imaging). 

Real-Time Cell-by-Cell Analysis with Agilent xCELLigence RTCA eSight 

Combining Live Cell Imaging with Cellular Impedance to Monitor Apoptotic Cell Death in Real Time 

Live Cell Analysis of Scratch Wound Migration and Invasion Healing Assays using the Agilent xCELLigence RTCA eSight 

Phagocytosis 

Dynamically analyze key mechanisms by which the immune system removes pathogens and cell debris. Track the entire process in real-time by imaging, screen for pathway modulators, and discover therapeutic interventions. Label and visualize macrophages via nuclear labeling reagents. 

Real-Time Visualization and Quantitative Analysis of Macrophage Phagocytosis Using the xCELLigence RTCA eSight 

BioTek Cytation 10 Confocal Imaging Reader

Confocal plus widefield = stunning images and analysis 

Cytation C10 captures stunning detail in a wide variety of sample types. Use widefield imaging for faster acquisition of large samples at lower magnification, switch to confocal to image small intracellular details or 3D samples. Or combine both modes for highly multiplexed, multiparameter imaging experiments. 

High-Throughput Platform for Fluorescence Image-Based Neurite Outgrowth Analysis 

Efficacy of a Novel Confocal Imaging Reader for Evaluating Bacterial Biofilms 

Histology and Slide Scanning for Light Microscopy 

Detailed Characterization of Mitosis Using Cell Population Analysis and Auto ROI Defined Confocal Imaging 

Environmental controls for live cell imaging 

Successful live cell kinetic imaging relies on a consistent environment, including temperature control and CO2/O2 control and monitoring. Cytation C10 provides the perfect environment to grow and analyze live cells over time. Powerful movie maker and kinetic analysis software tools allow visualizing and analysis time-lapse experiments. 

Quantification of Viral Infections Using AI/ML Analysis with Brightfield Imaging 

Automated Label-Free Imaging Assay for Measuring T Cell Activation and Cluster Formation 

Automated Assay for Measuring CAR T Cell Killing in 3D Cancer Models 

Rapid, Image-Based Viability and Outgrowth Analysis for Neurotoxicity Assays 

Hit-picking: Imaging + multimode detection saves time and data storage 

Imaging data sets can take a long time to acquire and require large data storage capacity. The unique hit-picking function takes advantage of the embedded plate reader optics. Set the hit picking criteria, quickly prescreen the microplate with the plate reader optics and Cytation C10 will automatically image the samples that meet your criteria, saving both time and hard disk space. 

Quantitative Assessment of Antioxidant Activities in Living Cells Using Cytation 5 Cell Imaging Multimode Reader 

Cell Viability Assessment by LUCS Assay 

Dako Autostainer Link 48

Manage all your slides with one solution 

Process 48 slides in less than three hours. This makes it possible to finalize 96 slides during a regular working day with only one Autostainer Link 48 and one PT Link. Gain up to 45 minutes of your run time compared to our previously fastest Autostainer - Autostainer Plus. Get the most out of your laboratory time by processing slides in parallel using PT Link and the fastest ever Autostainer Link 48. Have the freedom to set up your own standards and a possibility to control these 

Optimized Immunohistochemistry Workflow Facilitated by New Dako Autostainer Link 48 Software 

NovoCyte Opteon Flow Cytometer

Immunophenotyping 

There is a significant need for methodologies and instruments that provide high-throughput, in-depth analyses of the immune system at the single-cell level. Contrary to conventional cytometry, which is limited to as many fluorescence parameters as there are detectors, spectral cytometry is able to capture the full fluorescence spectrum for every marker across all laser lines, allowing for more parameters in a single experiment. 

In-Depth Characterization of the Major Leukocyte Cell Subsets in Human Peripheral Mononuclear Cells 

 Small Particle Detection 

The NovoCyte Opteon FSC/SSC detection optics and signal processing electronics have been optimized with dual 488 nm-SSC and 405 nm-SSC to resolve particles as small as 80 nm without adjusting settings to view larger cells in the same sample. With this, high-resolution platelets, bacteria, and various sub-micron particles can be readily identified and analyzed alongside your cell subsets. 

Capability of Spectral Flow Cytometry for Resolving Fluorochromes with Highly Overlapping Spectra 

Dual SSC to Separate RBCs from WBCs 

The optimized dual 488 nm-SSC and 405 nm-SSC on the NovoCyte Opteon can be used to separate red blood cells (RBCs) from white blood cells (WBCs) and platelets without the need for any RBC lysis treatment. 

Biotek Synergy H1 Multimodal Plate Reader

Extended dynamic range 

Synergy H1 offers an extended dynamic range, which allows detection of signals across a 7-log measurement range, which is useful for applications including enzyme kinetics, growth curves and end point assays with a large dynamic range or high expressors. A shorter dynamic range can cause reduced sensitivity on the low end or saturated signals on the high end of the assay signal range. 

UV Fluorescence Polarization as a Means to Investigate Protein Conformational and Mass Change 

HTRF Ligand Binding Assay for the Chemokine Receptor CXCR4 

BioTek Resources for Agilent pH-Xtra Glycolysis Assay 

BioTek Resources for Agilent MitoXpress Intra Intracellular Oxygen Assay 

BioTek Resources for Agilent MitoXpress Xtra Oxygen Consumption Assay 

Automated Non-Radioactive Assay Methods For ADCC and CDC Assays 

Multi-Volume Based Protein Quantification Methods 

Synergy Microplate Readers and HTRF Detection 

Environmental controls for cell-based assays 

Temperature control to 70 °C, CO2/O2 control, and shaking create the ideal environment for live cell assay workflows. A consistent environment leads to consistent data for long-term kinetic assays. 

Monitoring Growth of Beer Brewing Strains of Saccharomyces Cerevisiae 

Dual syringe injectors with specialized tips 

The robust precise dual syringe design eliminates the need for regular tubing replacement required by some peristaltic injector designs. Synergy H1 offers two tip types; straight tips that enable vigorous mixing for rapid inject/read assays, and the angled tip option that won't disturb cell layers for applications such as calcium kinetics. 

Validation and Comparison of Single-Step Flash and Dual-Spectral Luciferase Reporter Gene Assays using the Synergy Line of Microplate Readers 

xCelligence RTCA DP

 Cell Invasion and Migration Assays 

Cell invasion and migration is critical to many facets of biology, including processes such as wound healing and cancer cell metastasis. A major hurdle to studying cell invasion and migration is the lack of techniques that are quantitative, reproducible, and efficient. The xCELLigence RTCA DP system collects migratory data over extended periods with no back-end sample processing—there is no need to disassemble the unit and stain and count the cells, and the RTCA software creates the kinetic migration curves automatically. 

Using the xCELLigence RTCA DP Instrument to Perform Cell Invasion and Migration (CIM) Assays 

xCELLigence Portfolio Cancer Immunotherapy Handbook 

1290 Infinity II Bio LC/TQ System

An End-to-End Targeted Metabolomics Workflow 

Targeted metabolomics aims to routinely detect and quantify a defined group of metabolites. The 6495D LC/TQ is well suited for this role, as it can acquire reproducible and sensitive data at low dwell times—allowing you to analyze hundreds of analytes in a single chromatographic run. Combined with other solutions such as the 1290 Infinity III Bio LC system, HILIC-Z chromatography, Bravo Metabolomics sample preparation, Captiva EMR, and Mass Profiler Professional, the user can execute an end-to-end workflow for powerful biological insights. 

An End-to-End Targeted Metabolomics Workflow 

Population Analysis of the Plasma Lipidome 

The analytical sensitivity, speed, and robustness of the 6495D triple quadrupole LC/MS enables users to measure hundreds of different lipid species within a short analytical timeframe with high degree of specificity. These benefits help to map the lipidome in increasing detail. The most exciting application of these techniques is the study of lipid molecules at the population level, revealing association between lipid species, biochemical pathways, and biological state. Read more about a method for the detailed analysis of the plasma lipidome. 

An Inter-Laboratory Evaluation of a Targeted Lipidomics Method in Plasma 

Quantitative Proteomics Using Standard- or Nanoflow HPLC 

The 6495D LC/TQ with Jet Stream ionization source is a powerful standard-flow LC/MS with outstanding performance for targeted peptide quantification due to its ultrahigh sensitivity, reproducibility, and robustness. It has been used in plasma protein derived peptide analysis, and it has been demonstrated that this system reaches detection limits of nanoflow/nano-ESI. 

Comprehensive Protein Quantification in Plasma with Agilent 6495 Triple Quad LC/MS 

Robust and Reproducible Protein Quantification in Plasma using the Evosep One and the Agilent 6495 Triple Quadrupole LC/MS 

Quantification of Monoclonal Antibody Trastuzumab in Mouse Plasma with the Agilent 1290 Infinity II Bio LC and Agilent 6495 Triple Quadrupole LC/MS Systems 

Quantification of Therapeutic Peptide Exenatide in Rat Plasma Using Agilent Infinity II Bio LC and Agilent 6495 Triple Quadrupole LC/MS Systems 

Environmental and Pharmaceutical Testing 

Intelligent Reflex Fast Screening for Drugs in Urine 

Simultaneous Estimation of Eleven Nitrosamine Impurities in Metformin Drug Product Using an Agilent 6495D LC/TQ 

Determination of Nitrosamine Impurities Using the Agilent 6495 Triple Quadrupole LC/MS System 

A Comprehensive Workflow for Analysis of PFAS in Wastewater per EPA Method 1633